Enterotrophotic
Enterotrophotic is a scientific blend of Bacilluscereussensulato (MCCB 101) (Genbank accession. no. EF 062509 and Arthrobacter nicotianae(Genbank accession no. EU402968).(MCCB 104) isolated from culture environment and characterized for application as gut probiotic. They control Vibrio in shrimp/prawn intestine by way antagonistic activity mediated by an extracellular anti-vibrio molecule and enhances feed acceptance and digestion by way of producing hydrolytic enzymes.The dosage has been calculated to make available each animal 1×103 CFU per animal on daily basis and it would be 20ml per Kg feed.Dilute the calculated quantity of Enterotrophotic with an appropriate binder gently mix with feed, shade dry for one hour and administer to the animal in such a way that the probiotic will be available to all animals.
Dosage
The dosage has been calculated to make available each animal 1×103 CFU per animal on daily basis.
| No of Animals | Day of Culture | Dosage per day required |
|---|---|---|
| For every 10000 animals | 0-70 | 50 ml |
| For every 10000 animals | 70-120 | 100 ml |
Application
Dilute the calculated quantity of “Enterotrophotic” with sufficient quantity of water, sprinkle over feed and mix gentle, shade dry for 30 minutes and administer to the animal. If the animals are fed three times a day Enterotrophotic should be split in to three portions and added accordingly so that the probiotic will be available for all the animals always.
Example: Calculation of dosage of Enterotrophotic
| Initial stocking | 100,000 |
| Survival | 70% i.e. 70,000 animals |
| Enterotrophotic needed for 10,000 animals for 0-70 days | 50ml per day |
| Enterotrophotic needed for 70,000 animals for 0-70 days | (50/10,000) x 70,000 = 350ml per day |
| Enterotrophotic needed for 10,000 animals for 70- 120 days | 100 ml per day |
| Enterotrophotic needed for 70,000 animals for 70- 120days | (100/10,000) x 70,000 = 700 ml per day |