Dr. Jayesh Puthumana

Assistant Professor

Dr. Jayesh Puthumana received his Ph.D. in Cellular and molecular biology (marine biotechnology) (2013) and M.Sc. in Environmental Biotechnology (1999) from the Cochin University of Science and Technology. Dr. Jayesh joined the NCAAH, CUISAT in 2017 as Assistant professor in marine biotechnology. Dr. Jayesh was a Brain Korea fellow (2016-2017) and did his Postdoctoral research at Sungkyunkwan University, Suwon, Korea. He was a recipient of Innovative Young Biotechnology Award (IYBA), Department of biotechnology, Govt. of India in the year 2015. Besides, he was a visiting scholar/scientist at Centre for Marine Biomedicine and Biotechnology, Scripps Institutions of Oceanography (SIO), University of California at San Diego, USA and Virginia Tech, Hampton, Virginia, USA. Dr. Jayesh participated in Indian Arctic scientific expedition in 2015 and worked on the Arctic viral metagenomics.

Dr. Jayesh Puthumana has 12 years of research experience in marine biotechnology particularly in the fields and cell and molecular biology. After joining as an Assistant professor in marine biotechnology at NCAAH in 2017, the research activities of Dr. Jayesh have been focused on genome editing and genetic improvement of animals in addition to Induced pluripotent stem cells (iPSC), functional genomics, antibody therapeutics, and molecular and mechanistic toxicology.

Awards/Fellowships/Scholarships

  • Certificate of Appreciation from Virginia Tech, USA for presenting “RAS in India” during Fish School work shop at Virginia Tech/Virginia State University on 10th July 2018.
  • Achievement Award-2017, Cochin University of Science and Technology, , Kerala, India.
  • Brain Korea 21+ (BK21+) Post doctoral Research award 2016, Govt. of Korea.
  • Innovative Young Biotechnologist Award (IYBA)-2015, DBT, Govt of India.
  • Best Paper Award (Poster) TaSBE- 2016 (International Conference).
  • Dr. S. Kothari Post-doctoral Fellowship (UGC-DSKPDF) 2013-2016 from University Grants Commission, Govt. of India.
  • Research Associateship (RA) 2012-2015 from the Department of Biotechnology (DBT), Govt. of India.
  • Research Associateship (RA) 2012-2015 from the Department of Biotechnology (DBT), Govt. of India.
  • Senior Research Fellowship (SRF) 2009-2010 from the DBT, Govt. of India.
  • Junior Research Fellowship (JRF) 2007-2009 from the DBT, Govt. of India.
  • Qualified National Eligibility Test (NET) in 2007 by University Grant Commission, New Delhi.
  • University (CUSAT) First Rank holder in M. Sc. Environmental Biotechnology (Reg. No. 25040006, May 2006).
  • Qualified National Eligibility Test (NET) in 2005 by University Grant Commission, New Delhi. (Reg. No. V 163273).
  • Gold medalist, M.Sc, Cochin University of Science and Technology, Kerala, India.
  • Merit Scholarship holder throughout the University education.
  • Received best student award during graduation in the year 2004

Teaching and academic activities

At M.Tech. Level (Marine Biotechnology)

  1. Cell and Hybridoma Technology
  2. Model Systems, Molecular Genetics and Genetic Engineering
  3. Genetic improvement for High Health Brood Stock
  4. Marine Microbiology
  5. Skill Development in Model Systems, Molecular Genetics and Genetic Engineering (Lab).
  6. Skill Development in Cell culture and Hybridoma/Antibody Technology (Lab)
  7. Skill Development in Biotechnological interventions in Aquatic Animal health management (Lab)
  8. Skill Development in Molecular diagnostics and therapeutics/health management strategies


Professional Experience

  • Asst. Professor, NCAAH, CUSAT Nov 2017- Present
  • Visiting Scholar/Scientist Virginia Tech: Hampton, Virginia, USA 1st July -30th July 2018
  • Visiting Scholar/Scientist University of California San Diego Scripps Institution of Oceanography: La Jolla, CA, USA 2nd June 30th June 2018
  • Fellow DBT-IYBA, NCAAH, CUSAT March 2017- Present
  • Postdoctoral Scientist (BK21+),Dept. of Biological Science, College of Science, Sungkyunkwan University, Suwon, Korea, June 2016- March 2017
  • Postdoctoral Fellow (UGC-DSKPDF), School of Marine Sciences, CUSAT, June 2013 – 2016
  • Field Investigator in Indian Arctic Scientific Expedition 2015-2016 at Ny-Ålesund, Svalbard, 79oN-Arctic. June 2015- July 2015
  • Research Associate, NCAAH, CUSAT Nov 2012 – June 2013
  • Senior Research Fellow, NCAAH, CUSAT Oct 2009 – Oct 2012
  • Junior Research Fellow, NCAAH, CUSAT Oct 2007 – Oct 2009
  • Consultant, School of Environmental Studies, CUSAT, India Sept 2006 – Oct 2007
  • Research Associate, The Energy and Resources Institute (TERI), New Delhi, India June–Aug 2006 

Research Interests

Genome editing and genetic improvement in fishes, Cell line and stem cell development, Induced pluripotent stem cells (iPSC), functional genomics, antibody therapeutics, and molecular and mechanistic toxicology.

Projects Undertaken

Ongoing Research Projects

Development of Induced Pluripotent Stem Cells (iPSC) from Daphnia pulex and neural tissue engineering for the identification of genetic and epigenetic factors responsible for differentiation and neural degeneration.

Funding Agency: Department of Biotechnology

 

Optimization of Genome Editing for Loss-of-function study in Danio rerio-DRE/DRF cell line using RNA-guided Cas9 nuclease

Funding Agency: UGC-SMNRI

Development of CRISPR/Cas9 engineering platform for genome editing in Danio rerio and generation of mstn-knock-out lines

Funding Agency: University Grants Commission

Development of Shrimp anti-WSSV-IgY Antibody Therapeutics (WSSV-IgY-T) from immunoglobulin Y (IgY) using WSSV Antigens and Synthetic Peptides and their Commercial Applications in Aquaculture Industries

Funding Agency: DST-SERB

Publications

Book published (1)

  1. Jayesh P and I.S Bright Singh (2014) Advances in the Development of Shrimp Cell Lines ISBN 978-93-80095-54-7. Directorate of Public Relations and Publications, CUSAT, India

Book edited (1)

  1. Hatha MAA, Jayesh P, Rahiman MKM, Jabir T, Abhijith M, Joseph AC (2015) Climate Change and Marine Ecosystems- ISBN 978-81-922264-1-
  1. Seena Jose, Jayesh P, Rosamma Philip, A. Mohandas, and I.S. Bright Singh. Shrimp Cell Culture: A review (2007) Aquaculture and Marine Biotechnology. ISBN 978-81-900724-2-0 Pp 76-89.
  1. Balakrishnan, S., Singh, I.S.B., Puthumana, J., 2022. Status in molluscan cell line development in last one decade (2010–2020): impediments and way forward. Cytotechnology. https://doi.org/10.1007/s10616-022-00539-x [in press] view
  2. Ajitha, V., Sarasan, M., Parvathi Sreevidya, C., Aswathy, C., Kachiprath, B., Mohandas, A., Singh, I.S.B., Hameed, A.S.S., Schlenk, D., Magnuson, J.T., Puthumana, J., 2021a. Cytotoxic impacts of treated electroplating industrial effluent and the comparative effect of their metal components (Zn, Hg, and Zn+Hg) on Danio rerio gill (DrG) cell line. Sci. Total Environ. 793, 148533. https://doi.org/10.1016/j.scitotenv.2021.148533 view
  3. Ajitha, V., Sreevidya, C.P., Sarasan, M., Park, J.C., Mohandas, A., Singh, I.S.B., Puthumana, J., Lee, J.-S., 2021b. Effects of zinc and mercury on ROS-mediated oxidative stress-induced physiological impairments and antioxidant responses in the microalga Chlorella vulgaris. Environ. Sci. Pollut. Res. 28, 32475–32492. https://doi.org/10.1007/s11356-021-12950-6 view
  4. Anoop, B.S., Puthumana, J., Vazhappilly, C.G., Kombiyil, S., Philip, R., Abdulaziz, A., Bright Singh, I.S., 2021. Immortalization of shrimp lymphoid cells by hybridizing with the continuous cell line Sf9 leading to the development of ‘PmLyO-Sf9 .’ Fish Shellfish Immunol. 113, 196–207. https://doi.org/10.1016/j.fsi.2021.03.023 view
  5. B.S., A., Puthumana, J., Sukumaran, V., Vazhappilly, C.G., Kombiyil, S., Philip, R., Singh, I.S.B., 2021. A Novel Approach of Transducing Recombinant Baculovirus into Primary Lymphoid Cells of Penaeus monodon for Developing Continuous Cell Line. Mar. Biotechnol. 23, 517–528. https://doi.org/10.1007/s10126-021-10043-6 view
  6. Bhaskaran Sathyabhama, A., Puthumana, J., Kombiyil, S., Philip, R., Bright Singh, I.S., 2021. ‘PmLyO-Sf9 – WSSV complex’ could be a platform for elucidating the mechanism of viral entry, cellular apoptosis and replication impediments. Virology 553, 102–110. https://doi.org/10.1016/j.virol.2020.10.014 view
  7. Manomi S, Neema J, Jayesh P, Ravinesh R, Prabhakaran MP, Lathika CT, Rosamma P (2020) Exploration and profiling of hidden endophytic mycotaof marine macroalgae with potential drug leads. FEMS Microbiology Letters, 367, fnaa078
  8. Ajitha, V., Sreevidya, C.P., Kim, J.H., Bright Singh, I.S., Mohandas, A., Lee, J.-S., Puthumana, J., 2019. Effect of metals of treated electroplating industrial effluents on antioxidant defense system in the microalga Chlorella vulgaris. Aquat. Toxicol. 217, 105317. https://doi.org/10.1016/j.aquatox.2019.105317 view
  9. Preena, P.G., Achuthan, C., Kumar, V.J.R., Boobal, R., Deepa, G.D., Puthumana, J., Poulose, S., Surekhamol, I.S., Singh, I.S.B., 2019. Community composition of marine and brackish water ammonia-oxidizing consortia developed for aquaculture application. Water Sci. Technol. 79, 1017–1028. https://doi.org/10.2166/wst.2019.029 view
  10. Sruthy, K.S., Nair, A., Antony, S.P., Puthumana, J., Singh, I.S.B., Philip, R., 2019a. A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization. Fish Shellfish Immunol. 92, 667–679. https://doi.org/10.1016/j.fsi.2019.06.044 view
  11. Sruthy, K.S., Nair, A., Antony, S.P., Puthumana, J., Singh, I.S.B., Philip, R., P, J., Singh, I.S.B., Philip, R., Puthumana, J., Singh, I.S.B., Philip, R., 2019b. A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization. Fish Shellfish Immunol. 92, 667–679. https://doi.org/10.1016/j.fsi.2019.06.044 view
  12. Kachiprath, B., Puthumana, J., Gopi, J., Solomon, S., Krishnan, K.P., Philip, R., 2018. Amplicon sequencing based profiling of bacterial diversity from Krossfjorden, Arctic. Data Br. https://doi.org/10.1016/j.dib.2018.11.101 view
  13. Mohan, A.S., Nusaira, P., Raju, R., Puthumana, J., Kachiprath, B., Solomon, S., Jayanath, G., Philip, R., 2018. Bacterial Communities from near Glacier and Transition Zones of Kongsfjorden, Arctic: A Metagenomic Study. Indian J. Sci. Res. 20, 56–62 view
  14. Divya Jose, Jayesh P, Prem Gopinath, A Mohandas, I S Bright Singh (2017). A rapid two step bacterial DNA extraction method using LasA protease of Pseudomonas aeruginosa MCCB 123. Indian Journal of Biotechnology 16:425-434 .
  15. Jayesh P, Kim B.-M, Jeong C.-B, Kim D.-H, Kang H.-M, Han J, Lee J.-S, (2017). Nine co-localized cytochrome P450 2N, 2AD and 2P gene family in the genome of mangrove killifish Kryptolebias marmoratus: Identification and expression in response to B[α] P, BPA, OP, and NP. Aquat. Toxicol. 187, 132-140. (I F – 4.13)
  16. Jayesh P, Lee M-C, Park JC, Kim H.-S, Hwang D-S, Han J, Lee J-S (2017). Ultraviolet B radiation induces impaired lifecycle traits and modulates expression of cytochrome P450 (CYP) genes in the copepod Tigriopus japonicus. Aquat. Toxicol. 184, 116-122. (I F – 4.13)
  17. Jayesh P, Lee M-C, Han J, Kim, H-S Hwang D-S, Lee J-S. (2017). Ecdysone receptor (EcR) and ultraspiracle (USP) genes from the cyclopoid copepod Paracyclopina nana: Identification and expression in response to water accommodated fractions (WAFs). Comp. Biochem. Physiol., Part C 192, 7–15. (I F – 2.7)
  18. Lee S-H, Lee M-C, Jayesh P, Park JC, Kang S, Han J, Shin K-H, Park HG, Souissi S, Om A-S, Lee J-S (2017). Effects of temperature on growth and fatty acid synthesis in the cyclopoid copepod Paracyclopina nana. Fish Sci. DOI 10.1007/s12562-017-1104-2. (IF – 0.93)
  19. Manomi S, Jayesh P*, Neema J, Han J, Lee J.-S, Philip R. (2017). Marine algicolous endophytic fungi- A promising drug resource of the era. J. Microbiol. Biotechnol. doi: 10.4014/jmb.1701.01036. (IF – 1.98)
  20. Augustine A, Kumaran J, Jayesh P, Sabu S, Singh ISB, Joseph V, (2017). Multifactorial interactions and optimization in biomass harvesting of marine picoalga Picochlorum maculatum MACC3 with different flocculants. Aquaculture 474, 18–25.
  21. Lee S-H, Lee M-C, Jayesh P*, Park JC, Kang S, Hwang D-S., Shin K-H, Park HG, Souissi S, Om A-S, Lee J-S, Han J. (2017). Effects of salinity on growth, fatty acid synthesis, and expression of stress response genes in the cyclopoid copepod Paracyclopina nana. Aquaculture. 470, 182-189. (IF – 3.022)
  22. Han J, Kim D.-H, Seo JS, Kim IC, Nelson DR, Jayesh P#, Lee J-S. (2017). Assessing the identity and expression level of the cytochrome P450 20A1 (CYP20A1) gene in the BPA, BDE-47, and WAF-exposed copepods Tigriopus japonicus and Paracyclopina nana. Comp. Biochem. Physiol., Part C. 193, 42–49. (IF – 2.7)
  23. Hwang D-S, Lee M-C, Kyung D-H, Kim H-S, Han J, Kim I-C, Jayesh P#, Lee J-S (2017). WAFs lead molting retardation of naupliar stages with down-regulated expression profiles of chitin metabolic pathway and related genes in the copepod Tigriopus japonicus. Comp. Biochem. Physiol., Part C 193, 9–17. (IF – 2.7)
  24. Jayesh, P., Vrinda, S., Priyaja, P., Philip, R., Singh, I.S.B., 2016. Impaired telomerase activity hinders proliferation and in vitro transformation of Penaeus monodon lymphoid cells. Cytotechnology 68, 1301–1314. https://doi.org/10.1007/s10616-015-9890-9 view
  25. Kim D-H, Jayesh P*, Kang H-M, Lee M-C, Jeong C-B, Han J, Hwang D-S, Kim I-C, Lee J-W, Lee J-S (2016). Adverse effects of MWCNTs on life parameters, antioxidant systems, and activation of MAPK signaling pathways in the copepod Paracyclopina nana. Aquatic Toxicology 179, 115-124.
  26. Priyaja, P., Jayesh, P., Haseeb, M., Jose, B., Philip, R., Singh, I.S.B., 2016a. Evaluation of pyocyanin toxicity in various life stages of Penaeus monodon and in nitrifying bacterial consortia for their safe application in recirculating aquaculture systems (RAS) to abrogate pathogenic vibrios. Aquac. Int. https://doi.org/10.1007/s10499-016-0075-0 view
  27. Priyaja, P., Jayesh, P., Philip, R., Bright Singh, I.S., 2016b. Pyocyanin induced in vitro oxidative damage and its toxicity level in human, fish and insect cell lines for its selective biological applications. Cytotechnology 68, 143–155. https://doi.org/10.1007/s10616-014-9765-5 view
  28. Puthumana, J., Philip, R., Bright Singh, I.S., 2016. Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters. Cytotechnology 68, 1147–1159. https://doi.org/10.1007/s10616-015-9872-y
  29. Han J, Jayesh P, Lee MC, Kim S, Lee J-S (2016). Different susceptibilities of the Antarctic and temperate copepods Tigriopus kingsejongensis and Tigriopus japonicus to ultraviolet B (UV-B) radiation. Marine Ecology Progress Series. 561, 99–107.
  30. Jayesh, Puthumana, Jose, S., Philip, R., Bright Singh, I.S., 2015. Cellular and molecular markers in monitoring the fate of lymphoid cell culture from Penaeus monodon Fabricius (1798). Fish Shellfish Immunol. 47, 893–901. https://doi.org/10.1016/j.fsi.2015.05.033 view
  31. Jayesh, P., Philip, R., Singh, I.S.B., 2015. Multifactorial interaction of growth factors on Penaeus monodon lymphoid cells and the impact of IGFs in DNA synthesis and metabolic activity in vitro. Cytotechnology 67, 559–571. https://doi.org/10.1007/s10616-014-9697-0 view
  32. Puthumana, J., Prabhakaran, P., Philip, R., Singh, I.S.B., 2015. Attempts on producing lymphoid cell line from Penaeus monodon by induction with SV40-T and 12S EIA oncogenes. Fish Shellfish Immunol. 47, 655–663. https://doi.org/10.1016/j.fsi.2015.08.010 view
  33. Wilson, W., Lowman, D.W., Antony, S.P., Jayesh, P., Bright Singh, I.S., Philip, R., 2015. Immune gene expression profile of Penaeus monodon in response to marine yeast glucan application and white spot syndrome virus challenge. Fish Shellfish Immunol. 43, 346–356. https://doi.org/10.1016/j.fsi.2014.12.032 view
  34. Priyaja P, Jayesh P, Correya NS, Sreelakshmi B, Sudheer NS, Philip R, Singh ISB (2014) Antagonistic effect of Pseudomonas aeruginosa isolates from various ecological niches on Vibrio species pathogenic to crustaceans. Journal of Coastal Life Medicine 2: 76-84
  35. Jose, D., Jayesh, P., Gopinath, P., Mohandas, A., Bright Singh, I.S., 2014. Potential application of beta-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction. Indian J. Exp. Biol. 52, 89–96. view
  36. Jayesh, P., Jose, S., Philip, R., Bright Singh, I.S., 2013. A novel medium for the development of in vitro cell culture system from Penaeus monodon. Cytotechnology 65, 307–322. https://doi.org/10.1007/s10616-012-9491-9 view
  37. Manjusha K, Jayesh P, Jose D, Sreelakshmi B, Priyaja P, Gopinath P, Saramma AV, Singh ISB (2013). Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture. Cytotechnology 65(2):199-212 (IF – 1.86)
  38. Jayesh, P., Seena, J., Bright Singh, I.S., 2012. Establishment of Shrimp Cell Lines: Perception and Orientation. Indian J. Virol. 23, 244–251. https://doi.org/10.1007/s13337-012-0089-9 view
  39. Jose, S., Jayesh, P., Sudheer, N.S., Poulose, G., Mohandas, A., Philip, R., Bright Singh, I.S., 2012. Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression. J. Fish Dis. 35, 321–334. https://doi.org/10.1111/j.1365-2761.2012.01348.x view
  40. Smitha S, Haseena VS, Narayanan TN, Mary APR, Ajayan PM, Jayesh P, Anas A A, Aburto RR, Mani SA, Joy PA, Anantharaman MR (2012) Fluorescent superparamagnetic iron oxide core–shell nanoprobes for multimodal cellular imaging. Materials Express. 2: 265-274. doi:10.1166/mex.2012.1083
  41. Jose, S., Jayesh, P., Mohandas, A., Philip, R., Bright Singh, I.S., 2011. Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides. Mar. Environ. Res. 71, 169–177. https://doi.org/10.1016/j.marenvres.2010.12.008 view
  42. Narayanan TN., Mary APR, Anas PKS, Kumar SD, Makarov D, Albrecht M, Jayesh P, Anas A, Anantharaman M. R (2011) Enhanced Bio-Compatibility of Ferrofluids of Self-Assembled Superparamagnetic Iron Oxide-Silica Core–Shell Nanoparticles. Journal of Nanoscience and Nanotechnology. 11: 1958–1967
  • Development of a novel cell culture medium SCCM (Shrimp Cell Culture Medium) exclusively for shrimp cell culture. Patent application submitted to DBT on 15.2.2012. (Inventors: Prof. I. S. Bright Singh; Dr. Rosamma Philip; Dr. Jayesh, P and Dr. Seena Jose). Assignee: National Centre for Aquatic Animal Health, Cochin University of Science and Technology and Department of Biotechnology, Government of India.

National / International Conferences –25

GenBank Submissions :

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