Dr. Jeswin Joseph

Chief Minister’s Nava Kerala Post Doctoral Fellow

RNAi of STAT gene to protect the shrimp from WSSV infection

In shrimp aquaculture, the main pathogen is white spot syndrome virus (WSSV), which cause collapse of the culture within 3-10 days post infection. As part of controlling this virus, WSSV entry pathway inside the host can be targeted. Signal transducers and activators of transcription (STATs) are a family of latent cytoplasmic transcription factors that comprise seven members in mammals. Upon activation by tyrosine phosphorylation, STATs can dimerize and translocate into the nucleus where they bind to their cognate DNA response elements and trigger gene transcription. In mammals, STATs are usually the common target of viruses. Previous studies have shown that WSSV can induce shrimp STAT activation to facilitate the transcription of the viral gene ie1, suggesting that the activated STAT was conducive to WSSV infection. In our study we are focusing on utilizing RNAi of STAT gene to inhibit the replication of WSSV.

Publications

  1. Karthika, R., George, I., Murugadas, V., Jeswin, J.,Sherin, P.S.A., Sanjeev, D., Visnuvinayagam, S., Manikanta, B., Prasad, M.M., Rao, B.M., 2021. Characterization of tailed phages against E. coli using DNA restriction digestion analysis. Fishery Technology 58, 53-58 view
  2. Jeong, J.M., Jeswin, J.,Bae, J.S.,Park, C.I., 2018. Differential expression of olive flounder (Paralichthysolivaceus) transcriptome during viral hemorrhagic septicemia virus (VHSV) infection at warmer and colder temperature. Data in Brief 19, 2023-2028 view
  3. Jeswin, J., Joo, M.S., Jeong, J.M., Bae, J.S., Choi, K.M., Cho, D.H., Park, S.I., Park, C.I., 2018. The first report of siglec-3/CD33 gene in a teleost (rock bream, Oplegnathusfasciatus): An analysis of its spatial expression during stimulation to red seabream iridovirus (RSIV) and two bacterial pathogens. Developmental and Comparative Immunology 84, 117-122 view
  4. Hwang, J.Y., Jeong, J.M., Kwon, M.G., Seo, J.S., Hwang, S.D., Son, M.H., Bae, J.S., Choi, K.M., Cho, D.H., Jeswin, J., Park, C.I., 2018. Olive flounder CD276 (B7-H3) a coinhibitory molecule for T cells: Responses during viral hemorrhagic septicemia virus (VHSV) stimulation. Fish and Shellfish Immunology73, 228-233 view
  5. Jeswin, J., Jeong, S.M., Jeong, J.M., Bae, J.S., Kim, M.C., Park, C.I., 2017. Molecular characterization of a T cell costimulatory receptor, CD28 of rock bream (Oplegnathus fasciatus): Transcriptional expression during bacterial and viral stimulation. Fish and Shellfish Immunology 66, 354-359 view
  6. Jeswin, J., Jeong, J.M., Shim, J.D., Bae, J.S., Park, C.I., 2017. Protective responses of two paralogs of granulocyte colony stimulating factor (GCSF) in rock bream, Oplegnathus fasciatus during bacterial and viral infection. Fish and Shellfish Immunology 65, 206-212 view
  7. Chen, R.Y., Shen, K.L., Chen, Z., Fan, W.W., Xie, X.L.,Meng, C., Chang, X.J., Zheng, L.B., Jeswin, J., Wang, K.J., Liu, H.P., 2016. White spot syndrome virus entry is dependent on multiple endocytic routes, recruits autophagy, and is strongly facilitated by the autophagy-related protein Cq-GABARAP in a CME-dependent manner. Scientific Reports 6, 28694 view
  8. Jeswin, J., Xie, X.L., Wang, K.J., Liu, H.P., 2016. Proteomic analysis by iTRAQ in hematopoietic stem cells from red claw crayfish Cherax quadricarinatus post infection of white spot syndrome virus. Fish and Shellfish Immunology 50, 288-296 view
  9. Anju, A.,Jeswin,J.,Thomas, P.C.,Vijayan, K.K., 2015.Messenger RNA expression of pattern recognition receptor proteins (PRP) in the pearl oyster Pinctada fucata in response to LPS stimulation. Indian Journal of Fisheries 62, 151-156 view
  10. Zheng, C.Q., Jeswin, J., Shen, K.L.,Lablche, M., Wang, K.J., Liu H.P., 2015. Detrimental effect of CO2-driven seawater acidification on a crustacean brine shrimp, Artemia sinica.Fish and Shellfish Immunology 43, 181-190 view
  11. Jeswin,J., Anju, A.,Thomas, P.C., Paulton, M.P., Vijayan, K.K., 2015. Analysis of viral load between different tissues and rate of progression of white spot syndrome virus (WSSV) in Penaeus monodon. Aquaculture Research 46, 2003-2012 view
  12. Anju, A.,Jeswin,J.,Thomas, P.C.,Vijayan, K.K., 2013. Molecular cloning, characterization and expression analysis of F-type lectin from pearl oyster Pinctada fucata. Fish and Shellfish Immunology35, 170-179 view
  13. Jeswin,J., Anju, A.,Thomas, P.C., Paulton, M.P., Vijayan, K.K., 2013. Survivability of Penaeus monodon during white spot syndrome virus infection and its correlation with immune related genes. Aquaculture 380-383, 84-90 view
  14. Jeswin,J., Anju, A.,Thomas, P.C., Paulton, M.P., Vijayan, K.K., 2013. Differential and additional expression of proteins in the subcellular organelles of Penaeus monodon (Fabricus) in response to white spot syndrome virus (WSSV) infection. Indian Journal of Fisheries 60, 99-106 view
  15. Anju, A.,Jeswin,J.,Thomas, P.C., Paulton, M.P., Vijayan, K.K.,2013. Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-Superoxide Dismutase (SOD) from pearl oyster Pinctada fucata. Fish and Shellfish Immunology 34, 946-950 view
  • Dong-He Cho, Jeswin Joseph, Ji-Min Jung, Gwang-Min Choi, Min-Soo Joo, Chan-II Park (2017). Characteristics and expression of T-cell surface antigen CD2 gene from rock bream (Oplegnathusfasciatus) and applicability as a marker. International symposium on Marine and agricultural genomics, Seoul, South Korea. April 19- 21, 2017. (Poster)
  • Jin-Sol Bae, Jeswin Joseph, Gwang-Min Choi, Dong-Hee Cho, Min-Soo Joo and Chan-II Park (2017). Functional characterization of two novel piscidins from rock bream (Oplegnathusfasciatus). International symposium on fisheries science for future generations, Tokyo, Japan. September 22 -24, 2017. (Poster)
  • Jeswin Joseph, M.P Paulton, P.C Thomas and K.K Vijayan (2011). Differential expression of immune related genes of tiger shrimp Penaeus monodon following experimental infection with analysis of White Spot Syndrome Virus (WSSV). Asia Pacific Aquaculture Conference organized by World Aquaculture Society and College of Fisheries, Kerala Agricultural University, India. January 17-21, 2011. (Oral)
  • Jeswin Joseph, M.P Paulton, P.C Thomas and K.K Vijayan (2011). Quantitative analysis of White Spot Syndrome Virus (WSSV) progression in experimentally infected tiger shrimp Penaeus monodon. Asia Pacific Aquaculture Conference organized by World Aquaculture Society and College of Fisheries, Kerala Agricultural University, India. January 17-21, 2011. (Poster)
  • Anju Antony,P.C Thomas, M.P Paulton, K.K Vijayan and Jeswin Joseph (2011). Expression analysis of genes involved in the biomineralization of Mebe pearl. Asia Pacific Aquaculture Conference organized by World Aquaculture Society and College of Fisheries, Kerala Agricultural University, India. January 17-21, 2011. (Poster)
  • Jeswin Joseph, M.P Paulton and P.C Thomas(2011).WSSV induced host protein expressional variations in tiger shrimp Penaeus monodon.Indian Fisheries Forum organized by Central Marine Fisheries Research Institute, India. December 19-23, 2011. (Poster)
Dr. Jeswin


Mentor

Prof. I. S. Bright Singh